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Using lsgui

Page history last edited by Max Seligman 3 years, 10 months ago

 

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The GUI consists of 3 windows. The console (lscon.m, Fig. 1) controls the camera and LEDs. Motion.m (Fig. 2) controls the x,y, and z motors on the LS720. The main GUI window, created by lsgui.m, Fig. 3, shows image data and statistics (max, min, and mean) in the side and bottom panes.

                    

 

 

Figures 1-3. lsgui uses 3 windows to control and render the output of the Lumascope, along with menus to select colormaps and special applications. The console remembers prior settings of each LED. Gain and exposure sliders respond logarithmically for cameras with extensive ranges. Fig. 3 left and bottom panes show minimum, mean, and maximum pixel values across the corresponding rows and columns of the image, in red, green, and blue respectively. A "+" labels the highest value of the mean and maximum. This image is streaming in RGB mode, with brightfield mapped to the red color plane, green fluorescence in green, and nothing in blue. In this H&E stained pathology specimen, the eosine fluorescence is prominent as green. The user has selected a rectangle to zoom in. When the mouse is let go, this rectangle will fill the principle axis.

 

Two modes of image streaming are supported. The Start Streaming button displays a live view with a single color excitation. The colormap can be changed via menu. Image statistics (left and bottom panes) update with each frame, at frame rates of ~5-15 frames per second (FPS) depending on the camera, region of interest (ROI), computer speed, etc. A second streaming mode is enabled by the adjacent Start RGB button, and since this mode is running in Fig. 3, the button label is changed to Stop RGB. This streaming mode displays multi-exposure images in pseudo color as specified by the pop-up menus on the bottom of lscon (Fig. 1). 

 

 

 

 

 

Under The Hood

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